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Interleukin-12p70 Expression by Dendritic Cells of HIV-1-Infected Patients Fails to Stimulate gag-Specific Immune Responses
Joint Authors
Van Tendeloo, Viggo F. I.
Vekemans, Marc
Vereecken, Katleen
Bracke, Lotte
Cools, Nathalie
Vanham, Guido
Van Gulck, Ellen
Atkinson, Derek
Berneman, Zwi N.
Source
Clinical and Developmental Immunology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A variety of immune-based therapies has been developed in order to boost or induce protective CD8+ T cell responses in order to control HIV replication.
Since dendritic cells (DCs) are professional antigen-presenting cells (APCs) with the unique capability to stimulate naïve T cells into effector T cells, their use for the induction of HIV-specific immune responses has been studied intensively.
In the present study we investigated whether modulation of the activation state of DCs electroporated with consensus codon-optimized HxB2 gag mRNA enhances their capacity to induce HIV gag-specific T cell responses.
To this end, mature DCs were (i) co-electroporated with mRNA encoding interleukin (IL)-12p70 mRNA, or (ii) activated with a cytokine cocktail consisting of R848 and interferon (IFN)-γ.
Our results confirm the ability of HxB2 gag-expressing DCs to expand functional HIV-specific CD8+ T cells.
However, although most of the patients had detectable gag-specific CD8+ T cell responses, no significant differences in the level of expansion of functional CD8+ T cells could be demonstrated when comparing conventional or immune-modulated DCs expressing IL-12p70.
This result which goes against expectation may lead to a re-evaluation of the need for IL-12 expression by DCs in order to improve T-cell responses in HIV-1-infected individuals.
American Psychological Association (APA)
Van Gulck, Ellen& Cools, Nathalie& Atkinson, Derek& Bracke, Lotte& Vereecken, Katleen& Vekemans, Marc…[et al.]. 2012. Interleukin-12p70 Expression by Dendritic Cells of HIV-1-Infected Patients Fails to Stimulate gag-Specific Immune Responses. Clinical and Developmental Immunology،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-452734
Modern Language Association (MLA)
Van Gulck, Ellen…[et al.]. Interleukin-12p70 Expression by Dendritic Cells of HIV-1-Infected Patients Fails to Stimulate gag-Specific Immune Responses. Clinical and Developmental Immunology No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-452734
American Medical Association (AMA)
Van Gulck, Ellen& Cools, Nathalie& Atkinson, Derek& Bracke, Lotte& Vereecken, Katleen& Vekemans, Marc…[et al.]. Interleukin-12p70 Expression by Dendritic Cells of HIV-1-Infected Patients Fails to Stimulate gag-Specific Immune Responses. Clinical and Developmental Immunology. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-452734
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452734