![](/images/graphics-bg.png)
Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis
Joint Authors
Leong, Ooi Kheng
Sulaiman, Shaida Fariza
Muhammad, Tengku Sifzizul Tengku
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Physalis minima L.
is reputed for having anticancer property.
In this study, the chloroform extract of this plant exhibited remarkable cytotoxic activities on NCI-H23 (human lung adenocarcinoma) cell line at dose- and time-dependent manners (after 24, 48 and 72 h of incubation).
Analysis of cell-death mechanism demonstrated that the extract exerted apoptotic programed cell death in NCI-H23 cells with typical DNA fragmentation, which is a biochemical hallmark of apoptosis.
Morphological observation using transmission electron microscope (TEM) also displayed apoptotic characteristics in the treated cells, including clumping and margination of chromatins, followed by convolution of the nuclear and budding of the cells to produce membrane-bound apoptotic bodies.
Different stages of apoptotic programed cell death as well as phosphatidylserine externalization were confirmed using annexin V and propidium iodide staining.
Furthermore, acute exposure to the extract produced a significant regulation of c-myc, caspase-3 and p53 mRNA expression in this cell line.
Due to its apoptotic effect on NCI-H23 cells, it is strongly suggested that the extract could be further developed as an anticancer drug.
American Psychological Association (APA)
Leong, Ooi Kheng& Muhammad, Tengku Sifzizul Tengku& Sulaiman, Shaida Fariza. 2011. Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-452739
Modern Language Association (MLA)
Leong, Ooi Kheng…[et al.]. Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-452739
American Medical Association (AMA)
Leong, Ooi Kheng& Muhammad, Tengku Sifzizul Tengku& Sulaiman, Shaida Fariza. Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-452739
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452739