Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis
Joint Authors
Ellison, Courtney K.
Whitman, William B.
Sarmiento, Felipe
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Coenzyme M is an essential coenzyme for methanogenesis.
The proposed biosynthetic pathway consists of five steps, of which the fourth step is catalyzed by sulfopyruvate decarboxylase (ComDE).
Disruption of the gene comE by transposon mutagenesis resulted in a partial coenzyme M auxotroph, which grew poorly in the absence of coenzyme M and retained less than 3% of the wild type level of coenzyme M biosynthesis.
Upon coenzyme M addition, normal growth of the mutant was restored.
Moreover, complementation of the mutation with the wild type comE gene in trans restored full growth in the absence of coenzyme M.
These results confirm that ComE plays an important role in coenzyme M biosynthesis.
The inability to yield a complete CoM auxotroph suggests that either the transposon insertion failed to completely inactivate the gene or M.
maripaludis possesses a promiscuous activity that partially complemented the mutation.
American Psychological Association (APA)
Sarmiento, Felipe& Ellison, Courtney K.& Whitman, William B.. 2013. Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis. Archaea،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-452753
Modern Language Association (MLA)
Sarmiento, Felipe…[et al.]. Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis. Archaea No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-452753
American Medical Association (AMA)
Sarmiento, Felipe& Ellison, Courtney K.& Whitman, William B.. Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis. Archaea. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-452753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452753