Resolvin D1 Reverts Lipopolysaccharide-Induced TJ Proteins Disruption and the Increase of Cellular Permeability by Regulating I κBα Signaling in Human Vascular Endothelial Cells
Joint Authors
Xie, Wanli
Yao, Shanglong
Sun, Wei
Gui, Ping
Wang, Huiqing
Wang, Linlin
Wang, Tingting
Yao, Chengye
Lin, Yun
Zhang, Xingcai
Wu, Qingping
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Tight Junctions (TJ) are important components of paracellular pathways, and their destruction enhances vascular permeability.
Resolvin D1 (RvD1) is a novel lipid mediator that has treatment effects on inflammatory diseases, but its effect on inflammation induced increase in vascular permeability is unclear.
To understand whether RvD1 counteracts the lipopolysaccharide (LPS) induced increase in vascular cell permeability, we investigated the effects of RvD1 on endothelial barrier permeability and tight junction reorganization and expression in the presence or absence of LPS stimulation in cultured Human Vascular Endothelial Cells (HUVECs).
Our results showed that RvD1 decreased LPS-induced increased in cellular permeability and inhibited the LPS-induced redistribution of zo-1, occludin, and F-actin in HUVECs.
Moreover, RvD1 attenuated the expression of IκBα in LPS-induced HUVECs.
The NF-κB inhibitor PDTC enhanced the protective effects of RvD1 on restoration of occludin rather than zo-1 expression in LPS-stimulated HUVECs.
By contrast, the ERK1/2 inhibitor PD98059 had no effect on LPS-induced alterations in zo-1 and occludin protein expressions in HUVECs.
Our data indicate that RvD1 protects against impairment of endothelial barrier function induced by LPS through upregulating the expression of TJ proteins in HUVECs, which involves the IκBα pathway but not the ERK1/2 signaling.
American Psychological Association (APA)
Zhang, Xingcai& Wang, Tingting& Gui, Ping& Yao, Chengye& Sun, Wei& Wang, Linlin…[et al.]. 2013. Resolvin D1 Reverts Lipopolysaccharide-Induced TJ Proteins Disruption and the Increase of Cellular Permeability by Regulating I κBα Signaling in Human Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-452808
Modern Language Association (MLA)
Zhang, Xingcai…[et al.]. Resolvin D1 Reverts Lipopolysaccharide-Induced TJ Proteins Disruption and the Increase of Cellular Permeability by Regulating I κBα Signaling in Human Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-452808
American Medical Association (AMA)
Zhang, Xingcai& Wang, Tingting& Gui, Ping& Yao, Chengye& Sun, Wei& Wang, Linlin…[et al.]. Resolvin D1 Reverts Lipopolysaccharide-Induced TJ Proteins Disruption and the Increase of Cellular Permeability by Regulating I κBα Signaling in Human Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-452808
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452808