Essential Role of Thioredoxin 2 in Mitigating Oxidative Stress in Retinal Epithelial Cells
Joint Authors
Tabata, Kitako
Sugano, Eriko
Tomita, Hiroshi
Takahashi, Maki
Tamai, Makoto
Murayama, Namie
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The retina is constantly subjected to oxidative stress, which is countered by potent antioxidative systems present in retinal pigment epithelial (RPE) cells.
Disruption of these systems leads to the development of age-related macular degeneration.
Thioredoxin 2 (Trx2) is a potent antioxidant, which acts directly on mitochondria.
In the present study, oxidative stress was induced in the human RPE cell line (ARPE-19) using 4-hydroxynonenal (4-HNE) or C2-ceramide.
The protective effect of Trx2 against oxidative stress was investigated by assessing cell viability, the kinetics of cell death, mitochondrial metabolic activity, and expression of heat shock proteins (Hsps) in Trx2-overexpressing cell lines generated by transfecting ARPE cells with an adeno-associated virus vector encoding Trx2.
We show that overexpression of Trx2 reduced cell death induced by both agents when they were present in low concentrations.
Moreover, early after the induction of oxidative stress Trx2 played a key role in the maintenance of the cell viability through upregulation of mitochondrial metabolic activity and inhibition of Hsp70 expression.
American Psychological Association (APA)
Sugano, Eriko& Murayama, Namie& Takahashi, Maki& Tabata, Kitako& Tamai, Makoto& Tomita, Hiroshi. 2013. Essential Role of Thioredoxin 2 in Mitigating Oxidative Stress in Retinal Epithelial Cells. Journal of Ophthalmology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-452819
Modern Language Association (MLA)
Sugano, Eriko…[et al.]. Essential Role of Thioredoxin 2 in Mitigating Oxidative Stress in Retinal Epithelial Cells. Journal of Ophthalmology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-452819
American Medical Association (AMA)
Sugano, Eriko& Murayama, Namie& Takahashi, Maki& Tabata, Kitako& Tamai, Makoto& Tomita, Hiroshi. Essential Role of Thioredoxin 2 in Mitigating Oxidative Stress in Retinal Epithelial Cells. Journal of Ophthalmology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-452819
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452819