Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells
Joint Authors
Xu, Hao
Wang, Shichao
Cui, Lifeng
Li, Meiying
Liu, Shutong
Chi, Guangfan
Li, Yu-Lin
Liu, Jin Yu
Xu, Jinying
Lv, Shuang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-18
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Bone marrow mesenchymal stem cells (BMSCs) transplants have been approved for treating central nervous system (CNS) injuries and diseases; however, their clinical applications are limited.
Here, we model the therapeutic potential of dermal papilla cells (DPCs) in vitro.
DPCs were isolated from rat vibrissae and characterized by immunocytofluorescence, RT-PCR, and multidifferentiation assays.
We examined whether these cells could secrete neurotrophic factors (NTFs) by using cocultures of rat pheochromocytoma cells (PC12) with conditioned medium and ELISA assay.
DPCs expressed Sox10, P75, Nestin, Sox9, and differentiated into adipocytes, osteoblasts, smooth muscle cells, and neurons under specific inducing conditions.
The DPC-conditioned medium (DPC-CM) induced neuronal differentiation of PC12 cells and promoted neurite outgrowth.
Results of ELISA assay showed that compared to BMSCs, DPCs secreted more brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF).
Moreover, we observed that, compared with the total DPC population, sphere-forming DPCs expressed higher levels of Nestin and P75 and secreted greater amounts of GDNF.
The DPCs from craniofacial hair follicle papilla may be a new and promising source for treating CNS injuries and diseases.
American Psychological Association (APA)
Li, Meiying& Liu, Jin Yu& Wang, Shichao& Xu, Hao& Cui, Lifeng& Lv, Shuang…[et al.]. 2014. Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-452867
Modern Language Association (MLA)
Li, Meiying…[et al.]. Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-452867
American Medical Association (AMA)
Li, Meiying& Liu, Jin Yu& Wang, Shichao& Xu, Hao& Cui, Lifeng& Lv, Shuang…[et al.]. Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-452867
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452867