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Immunosuppressive Factor MNSFβ Regulates Cytokine Secretion by Mouse Lymphocytes and Is Involved in Interactions between the Mouse Embryo and Endometrial Cells In Vitro
Joint Authors
Jia, Zhefu
Du, Yanbo
Jiang, Yahong
Shi, Yan
Salamonsen, Lois A.
Li, Zhuoya
He, Ya-Ping
Wang, Jian
Sun, Zhaogui
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Immune tolerance at the fetomaternal interface must be established during the processes of implantation and pregnancy.
Monoclonal nonspecific suppressor factor beta (MNSFβ) is a secreted protein that possesses antigen-nonspecific immune-suppressive function.
It was previously reported that intrauterine immunoneutralization of MNSFβ significantly inhibited embryo implantation in mice.
In the present study, MNSFβ protein expression was up- or downregulated by overexpression or RNA interference, respectively, in HCC-94 cells and the culture supernatants used to determine effects of MNSFβ on the secretion of IL-4 and TNFα from mouse lymphocytes as detected by ELISA.
A coculture model of mouse embryos and endometrial stromal cells was also utilized to determine the effects of a specific anti-MNSFβ antibody on hatching and growth of embryos in vitro.
The results show that MNSFβ induced secretion of IL-4 and inhibited secretion of TNFα from mouse lymphocytes.
Following immunoneutralization of MNSFβ protein in the HCC-94 supernatant, the stimulatory effect of MNSFβ on IL-4 secretion from mouse lymphocytes was reduced, while the inhibitory effect on secretion of TNFα was abrogated.
Expression of MNSFβ was detected in both embryonic and endometrial stromal cells, and its immunoneutralization inhibited the hatching and spreading of embryos in an in vitro coculture model.
These results indicated that MNSFβ may play critical roles during the peri-implantation process by regulating cytokine secretion of lymphocytes and by mediating the crosstalk between embryonic cells and endometrial stromal cells.
American Psychological Association (APA)
He, Ya-Ping& Sun, Zhaogui& Shi, Yan& Jiang, Yahong& Jia, Zhefu& Du, Yanbo…[et al.]. 2011. Immunosuppressive Factor MNSFβ Regulates Cytokine Secretion by Mouse Lymphocytes and Is Involved in Interactions between the Mouse Embryo and Endometrial Cells In Vitro. ISRN Immunology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-452898
Modern Language Association (MLA)
He, Ya-Ping…[et al.]. Immunosuppressive Factor MNSFβ Regulates Cytokine Secretion by Mouse Lymphocytes and Is Involved in Interactions between the Mouse Embryo and Endometrial Cells In Vitro. ISRN Immunology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-452898
American Medical Association (AMA)
He, Ya-Ping& Sun, Zhaogui& Shi, Yan& Jiang, Yahong& Jia, Zhefu& Du, Yanbo…[et al.]. Immunosuppressive Factor MNSFβ Regulates Cytokine Secretion by Mouse Lymphocytes and Is Involved in Interactions between the Mouse Embryo and Endometrial Cells In Vitro. ISRN Immunology. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-452898
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452898