LED Light-Activated Hypocrellin B Induces Mitochondrial Damage of Ovarian Cancer Cells
Joint Authors
Jiang, Yuan
Leung, Albert Wingnang
Xiang, Junyan
Xu, Chuanshan
Source
International Journal of Photoenergy
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-15
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Objective.
Hypocrellin is a natural photosensitizer from a traditional Chinese herb.
In the present study, our aim is to investigate the effect of LED light-activated hypocrellin B on mitochondria of ovarian cancer cells.
Material and Methods.
Ovarian cancer HO-8910 cells were incubated with hypocrellin B at the concentration of 2.5 μM for 5 h and then irradiated by blue light from a novel LED source.
Cell survival rate of HO-8910 cells was measured using MTT assay 24 h after photodynamic treatment of hypocrellin B.
Mitochondrial morphology was observed using transmission electron microscopy (TEM).
Mitochondrial membrane potential was measured using flow cytometry with JC-1 staining.
Results.
MTT assay showed that cell survival rate of HO-8910 cells in the photodynamic treatment group has significantly decreased down to 27.22±1.26% (P<0.01).
Light irradiation alone or hypocrellin B alone showed no significant impact.
In our TEM mitochondria of the cells after photodynamic treatment of hypocrellin B showed severe damage with swollen mitochondria that had nearly nonexistant cristae.
Mitochondrial membrane potential remarkably decreased after photodynamic action of hypocrellin B.
Conclusion.
The findings demonstrated that photodynamic action of hypocrellin B significantly decreased cell proliferation of ovarian cancer HO-8910 cells, caused severe damage to mitochondrial structure, and induced mitochondrial membrane collapse.
American Psychological Association (APA)
Jiang, Yuan& Leung, Albert Wingnang& Xiang, Junyan& Xu, Chuanshan. 2012. LED Light-Activated Hypocrellin B Induces Mitochondrial Damage of Ovarian Cancer Cells. International Journal of Photoenergy،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-452911
Modern Language Association (MLA)
Jiang, Yuan…[et al.]. LED Light-Activated Hypocrellin B Induces Mitochondrial Damage of Ovarian Cancer Cells. International Journal of Photoenergy No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-452911
American Medical Association (AMA)
Jiang, Yuan& Leung, Albert Wingnang& Xiang, Junyan& Xu, Chuanshan. LED Light-Activated Hypocrellin B Induces Mitochondrial Damage of Ovarian Cancer Cells. International Journal of Photoenergy. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-452911
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452911