Genome-Wide Uncovering of STAT3-Mediated miRNA Expression Profiles in Colorectal Cancer Cell Lines
Joint Authors
Wang, Ying
Deng, Ling
Zhang, Jufeng
Luo, Xia
Li, Huiming
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Colorectal cancer (CRC) is one of the most common malignancies resulting in high mortality worldwide.
Signal transducer and activator of transcription 3 (STAT3) is an oncogenic transcription factor which is frequently activated and aberrantly expressed in CRC.
MicroRNAs (miRNAs) are a class of small noncoding RNAs which play important roles in many cancers.
However, little is known about the global miRNA profiles mediated by STAT3 in CRC cells.
In the present study, we applied RNA interference to inhibit STAT3 expression and profiled the miRNA expression levels regulated by STAT3 in CRC cell lines with deep sequencing.
We found that 26 and 21 known miRNAs were significantly overexpressed and downexpressed, respectively, in the STAT3-knockdown CRC cell line SW480 (SW480/STAT3-siRNA) compared to SW480 transfected with scrambled siRNAs (SW480/siRNA-control).
The miRNA expression profiling was then validated by quantitative real-time PCR for selected known miRNAs.
We further predicted the putative target genes for the dysregulated miRNAs and carried out functional annotation including GO enrichment and KEGG pathway analysis for selected miRNA targets.
This study directly depicts STAT3-mediated miRNA profiles in CRC cells, which provides a possible way to discover biomarkers for CRC therapy.
American Psychological Association (APA)
Zhang, Jufeng& Luo, Xia& Li, Huiming& Deng, Ling& Wang, Ying. 2014. Genome-Wide Uncovering of STAT3-Mediated miRNA Expression Profiles in Colorectal Cancer Cell Lines. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-452951
Modern Language Association (MLA)
Zhang, Jufeng…[et al.]. Genome-Wide Uncovering of STAT3-Mediated miRNA Expression Profiles in Colorectal Cancer Cell Lines. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-452951
American Medical Association (AMA)
Zhang, Jufeng& Luo, Xia& Li, Huiming& Deng, Ling& Wang, Ying. Genome-Wide Uncovering of STAT3-Mediated miRNA Expression Profiles in Colorectal Cancer Cell Lines. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-452951
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452951