Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K
Joint Authors
Sourial, Samer
Cederbrant, Karin
Marcusson-Ståhl, Maritha
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The sensitivity of different renal regions to xenobiotics requires the development of a multiplex immunoassay for the simultaneous analysis of kidney biomarkers.
Calbindin D28K is a distal tubule-specific protein that can be detected in urine under pathological conditions.
In this study, a pair of anti-calbindin D28K antibodies was used in an immunoassay for the detection of calbindin D28K expression in rat and human kidney and urine.
Comparative analysis of the immunoassay was performed on the Meso Scale Development (MSD) and Luminex platforms.
Analysis on both platforms detected calbindin D28K concentrations between 100 ng/mL and 100 pg/mL.
Luminex detected 10-fold the amount of calbindin D28K in samples analyzed as compared to MSD, whereas calbindin D28K level in rat and human urine was below detection limit in both platforms.
The application of the immunoassays described herein may be useful in toxicological and pathological studies of distal tubular damage in rats and human.
American Psychological Association (APA)
Sourial, Samer& Marcusson-Ståhl, Maritha& Cederbrant, Karin. 2009. Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-5.
https://search.emarefa.net/detail/BIM-452982
Modern Language Association (MLA)
Sourial, Samer…[et al.]. Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-5.
https://search.emarefa.net/detail/BIM-452982
American Medical Association (AMA)
Sourial, Samer& Marcusson-Ståhl, Maritha& Cederbrant, Karin. Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-5.
https://search.emarefa.net/detail/BIM-452982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452982