In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP

Joint Authors

Gaume, Xavier
Bouvet, Philippe
Argoul, Françoise
Monier, Karine
Mongelard, Fabien

Source

Biochemistry Research International

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-15, 15 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-03-03

Country of Publication

Egypt

No. of Pages

15

Main Subjects

Chemistry

Abstract EN

Nucleolin is a major nucleolar protein involved in various aspects of ribosome biogenesis such as regulation of polymerase I transcription, pre-RNA maturation, and ribosome assembly.

Nucleolin is also present in the nucleoplasm suggesting that its functions are not restricted to nucleoli.

Nucleolin possesses, in vitro, chromatin co-remodeler and histone chaperone activities which could explain numerous functions of nucleolin related to the regulation of gene expression.

The goal of this report was to investigate the consequences of nucleolin depletion on the dynamics of histones in live cells.

Changes in histone dynamics occurring in nucleolin silenced cells were measured by FRAP experiments on eGFP-tagged histones (H2B, H4, and macroH2A).

We found that nuclear histone dynamics was impacted in nucleolin silenced cells; in particular we measured higher fluorescence recovery kinetics for macroH2A and H2B but not for H4.

Interestingly, we showed that nucleolin depletion also impacted the dissociation constant rate of H2B and H4.

Thus, in live cells, nucleolin could play a role in chromatin accessibility by its histone chaperone and co-remodeling activities.

American Psychological Association (APA)

Gaume, Xavier& Monier, Karine& Argoul, Françoise& Mongelard, Fabien& Bouvet, Philippe. 2011. In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP. Biochemistry Research International،Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-453001

Modern Language Association (MLA)

Gaume, Xavier…[et al.]. In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP. Biochemistry Research International No. 2011 (2011), pp.1-15.
https://search.emarefa.net/detail/BIM-453001

American Medical Association (AMA)

Gaume, Xavier& Monier, Karine& Argoul, Françoise& Mongelard, Fabien& Bouvet, Philippe. In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP. Biochemistry Research International. 2011. Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-453001

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-453001