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Comparative Assessment of Lymph Node Micrometastasis in Cervical, Endometrial and Vulvar Cancer : Insights on the Real Time qRT-PCR Approach versus Immunohistochemistry, Employing Dual Molecular Markers
Joint Authors
Markaki, Sofia
Christodoulou, Ioanna
Pappa, Kalliopi I.
Gazouli, Maria
Antsaklis, Aris
Rodolakis, Alexandros
Anagnou, Nicholas P.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To address the value of qRT-PCR and IHC in accurately detecting lymph node micrometastasis in gynecological cancer, we performed a systematic approach, using a set of dual molecular tumor-specific markers such as cytokeratin 19 (CK19) and carbonic anhydrase 9 (CA9), in a series of 46 patients (19 with cervical cancer, 18 with endometrial cancer, and 9 with vulvar cancer).
A total of 1281 lymph nodes were analyzed and 28 were found positive by histopathology.
Following this documentation, 82 lymph nodes, 11 positive and 71 negative, were randomly selected and further analyzed both by IHC and qRT-PCR for CK19 and CA9 expression.
All 11 (100%) expressed CK19 by IHC, while only 6 (54.5%) expressed CA9.
On the contrary, all the histologically negative for micrometastases lymph nodes were also negative by IHC analysis for both markers.
The comparative diagnostic efficacy of the two markers using qRT-PCR, however, disclosed that the analysis of the same aliquots of the 82 lymph nodes led to 100% specificity for the CK19 biomarker, while, in contrast, CA9 failed to recapitulate a similar pattern.
These data suggest that qRT-PCR exhibits a better diagnostic accuracy compared to IHC, while CK19 displays a consistent pattern of detection compared to CA9.
American Psychological Association (APA)
Pappa, Kalliopi I.& Rodolakis, Alexandros& Christodoulou, Ioanna& Gazouli, Maria& Markaki, Sofia& Antsaklis, Aris…[et al.]. 2014. Comparative Assessment of Lymph Node Micrometastasis in Cervical, Endometrial and Vulvar Cancer : Insights on the Real Time qRT-PCR Approach versus Immunohistochemistry, Employing Dual Molecular Markers. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-453007
Modern Language Association (MLA)
Pappa, Kalliopi I.…[et al.]. Comparative Assessment of Lymph Node Micrometastasis in Cervical, Endometrial and Vulvar Cancer : Insights on the Real Time qRT-PCR Approach versus Immunohistochemistry, Employing Dual Molecular Markers. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-453007
American Medical Association (AMA)
Pappa, Kalliopi I.& Rodolakis, Alexandros& Christodoulou, Ioanna& Gazouli, Maria& Markaki, Sofia& Antsaklis, Aris…[et al.]. Comparative Assessment of Lymph Node Micrometastasis in Cervical, Endometrial and Vulvar Cancer : Insights on the Real Time qRT-PCR Approach versus Immunohistochemistry, Employing Dual Molecular Markers. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-453007
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453007