Programmed Cell Death Induced by Modulated Electrohyperthermia

Abstract EN

Background.

Modulated electrohyperthermia (mEHT) is a noninvasive technique for targeted tumor treatment.

Method.

HT29 human colorectal carcinoma cell line xenografted to both femoral regions of BalbC/nu/nu mice was treated with a single shot OTM treatment.

Histomorphologic, immunohistochemical analysis TUNEL assay, and R&D Apoptosis array were performed on tissue samples.

Results.

mEHT caused a selective tumor demolition.

An upregulation of TRAIL-R2 and FAS was observed.

Cleaved caspase-3 positive cells appear at the tumor periphery.

Cytochrome c and AIF release was observed in line with massive TUNEL positivity.

Conclusion.

In HT29 colorectal cancer xenograft, mEHT caused massive caspase independent cell death.