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Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl− Secretion in Mammary Epithelia
Joint Authors
Ao, Mei
Sarathy nee Venkatasubramanian, Jayashree
Rao, Mrinalini C.
Devi, Y. Sangeeta
Krishnamra, Nateetip
Anantamongkol, Utchariya
Source
Journal of Signal Transduction
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-25
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Mammary gland ion transport is essential for lactation and is regulated by prolactin and glucocorticoids.
This study delineates the roles of prolactin receptors (PRLR) and long-term prolactin and dexamethasone (P-D)-mediation of [Ca2+]i and Cl− transport in HC-11 cells.
P-D (24 h) suppressed ATP-induced [Ca2+]i.
This may be due to decreased Ca2+ entry since P-D decreased transient receptor potential channel 3 (TRPC3) but not secretory pathway Ca2+-ATPase 2 (SPCA2) mRNA.
ATP increased Cl− transport, measured by iodide (I−) efflux, in control and P-D-treated cells.
P-D enhanced I− efflux response to cAMP secretagogues without altering Cl− channels or NKCC cotransporter expression.
HC-11 cells contain only the long form of PRLR (PRLR-L).
Since the short isoform, PRLR-S, is mammopoietic, we determined if transfecting PRLR-S (rs) altered PRLR-L-mediated Ca2+ and Cl− transport.
Untreated rs cells showed an attenuated [Ca2+]i response to ATP with no further response to P-D, in contrast to vector-transfected (vtc) controls.
P-D inhibited TRPC3 in rs and vtc cells but increased SPCA2 only in rs cells.
As in wild-type, cAMP-stimulated Cl− transport, in P-D-treated vtc and rs cells.
In summary, 24 h P-D acts via PRLR-L to attenuate ATP-induced [Ca2+]i and increase cAMP-activated Cl− transport.
PRLR-S fine-tunes these responses underscoring its mammopoietic action.
American Psychological Association (APA)
Anantamongkol, Utchariya& Ao, Mei& Sarathy nee Venkatasubramanian, Jayashree& Devi, Y. Sangeeta& Krishnamra, Nateetip& Rao, Mrinalini C.. 2012. Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl− Secretion in Mammary Epithelia. Journal of Signal Transduction،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-453285
Modern Language Association (MLA)
Anantamongkol, Utchariya…[et al.]. Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl− Secretion in Mammary Epithelia. Journal of Signal Transduction No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-453285
American Medical Association (AMA)
Anantamongkol, Utchariya& Ao, Mei& Sarathy nee Venkatasubramanian, Jayashree& Devi, Y. Sangeeta& Krishnamra, Nateetip& Rao, Mrinalini C.. Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl− Secretion in Mammary Epithelia. Journal of Signal Transduction. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-453285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453285