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DNA Methylation Changes during In Vitro Propagation of Human Mesenchymal Stem Cells : Implications for Their Genomic Stability?
Joint Authors
Tredici, Giovanni
Bentivegna, Angela
Riva, Gabriele
Foudah, Dana
Miloso, Mariarosaria
Dalprà, Leda
Butta, Valentina
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Mesenchymal stem cells (MSCs) hold great promise for the treatment of numerous diseases.
A major problem for MSC therapeutic use is represented by the very low amount of MSCs which can be isolated from different tissues; thus ex vivo expansion is indispensable.
Long-term culture, however, is associated with extensive morphological and functional changes of MSCs.
In addition, the concern that they may accumulate stochastic mutations which lead the risk of malignant transformation still remains.
Overall, the genome of human MSCs (hMSCs) appears to be apparently stable throughout culture, though transient clonal aneuploidies have been detected.
Particular attention should be given to the use of low-oxygen environment in order to increase the proliferative capacity of hMSCs, since data on the effect of hypoxic culture conditions on genomic stability are few and contradictory.
Furthermore, specific and reproducible epigenetic changes were acquired by hMSCs during ex vivo expansion, which may be connected and trigger all the biological changes observed.
In this review we address current issues on long-term culture of hMSCs with a 360-degree view, starting from the genomic profiles and back, looking for an epigenetic interpretation of their genetic stability.
American Psychological Association (APA)
Bentivegna, Angela& Miloso, Mariarosaria& Riva, Gabriele& Foudah, Dana& Butta, Valentina& Dalprà, Leda…[et al.]. 2013. DNA Methylation Changes during In Vitro Propagation of Human Mesenchymal Stem Cells : Implications for Their Genomic Stability?. Stem Cells International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-453308
Modern Language Association (MLA)
Bentivegna, Angela…[et al.]. DNA Methylation Changes during In Vitro Propagation of Human Mesenchymal Stem Cells : Implications for Their Genomic Stability?. Stem Cells International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-453308
American Medical Association (AMA)
Bentivegna, Angela& Miloso, Mariarosaria& Riva, Gabriele& Foudah, Dana& Butta, Valentina& Dalprà, Leda…[et al.]. DNA Methylation Changes during In Vitro Propagation of Human Mesenchymal Stem Cells : Implications for Their Genomic Stability?. Stem Cells International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-453308
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453308