NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes
Joint Authors
Zhao, Yanhua
Choudhary, Sanjeev
Banerjee, Srijita
Tilton, Ronald G.
LeJeune, Wanda S.
Source
Experimental Diabetes Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The impact of increased NF-κB-inducing kinase (NIK), a key component of the NF-κB activation pathways, on diabetes-induced renal inflammation remains unknown.
We overexpressed NIK wild type (NIKwt) or kinase-dead dominant negative mutants (NIKdn) in HK-2 cells and demonstrated that RelB and p52, but not RelA, abundance and DNA binding increased in nuclei of NIKwt but not NIKdn overexpressed cells, and this corresponded with increases in multiple proinflammatory cytokines.
Since TRAF3 negatively regulates NIK expression, we silenced TRAF3 by >50%; this increased nuclear levels of p52 and RelB, and transcript levels of proinflammatory cytokines and transcription factors.
In HK-2 cells and mouse primary proximal tubule epithelial cells treated with methylglyoxal-modified albumin, multiple proinflammatory cytokines and NIK were increased in association with increased nuclear RelB and p52.
These observations indicate that NIK regulates proinflammatory responses of renal proximal tubular epithelial cells via mechanisms involving TRAF3 and suggest a role for NF-κB noncanonical pathway activation in modulating diabetes-induced inflammation in renal tubular epithelium.
American Psychological Association (APA)
Zhao, Yanhua& Banerjee, Srijita& LeJeune, Wanda S.& Choudhary, Sanjeev& Tilton, Ronald G.. 2011. NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes. Experimental Diabetes Research،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-453328
Modern Language Association (MLA)
Zhao, Yanhua…[et al.]. NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes. Experimental Diabetes Research No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-453328
American Medical Association (AMA)
Zhao, Yanhua& Banerjee, Srijita& LeJeune, Wanda S.& Choudhary, Sanjeev& Tilton, Ronald G.. NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes. Experimental Diabetes Research. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-453328
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453328