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HSV-1 miR-H6 Inhibits HSV-1 Replication and IL-6 Expression in Human Corneal Epithelial Cells In Vitro
Joint Authors
Huang, Qiang
Wu, Kaili
Nie, Yuhong
Duan, Fang
Liao, Jingyu
Source
Clinical and Developmental Immunology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
HSV-1 infection in the cornea could lead to blindness.
The infected cell polypeptide 4 (ICP4) of herpes simplex virus 1 (HSV-1) is a regulator of viral transcription that is required for productive infection.
It has been previously demonstrated that miR-H6 encoded from HSV-1 genome targets ICP4 to help maintain latency.
In this study, synthesized miR-H6 mimics were transfected into HSV-1-infected human cornea epithelial (HCE) cells.
The inhibition of HSV-1 replication and viral ICP4 expression in miR-H6-transfected HCE was confirmed by plaque assay, immunofluorescence, and Western blot.
Compared to nontransfection or mock, miR-H6 produced a low-titer HSV-1 and weak ICP4 expression.
In addition, miR-H6 can decrease the interleukin 6 released into the medium, which was determined by ELISA.
Taken together, the data suggests that miR-H6 targeting of ICP4 inhibits HSV-1 productive infection and decreases interleukin 6 production in HCE, and this may provide an approach to prevent HSV-1 lytic infection and inhibit corneal inflammation.
American Psychological Association (APA)
Duan, Fang& Liao, Jingyu& Huang, Qiang& Nie, Yuhong& Wu, Kaili. 2012. HSV-1 miR-H6 Inhibits HSV-1 Replication and IL-6 Expression in Human Corneal Epithelial Cells In Vitro. Clinical and Developmental Immunology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-453354
Modern Language Association (MLA)
Duan, Fang…[et al.]. HSV-1 miR-H6 Inhibits HSV-1 Replication and IL-6 Expression in Human Corneal Epithelial Cells In Vitro. Clinical and Developmental Immunology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-453354
American Medical Association (AMA)
Duan, Fang& Liao, Jingyu& Huang, Qiang& Nie, Yuhong& Wu, Kaili. HSV-1 miR-H6 Inhibits HSV-1 Replication and IL-6 Expression in Human Corneal Epithelial Cells In Vitro. Clinical and Developmental Immunology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-453354
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453354