Separation and Identification of HSP-Associated Protein Complexes from Pancreatic Cancer Cell Lines Using 2D CNSDS-PAGE Coupled with Mass Spectrometry
Joint Authors
Liu, Hui
Li, Zhili
Wang, Xiaodong
Zhao, Zhiyun
Wang, Xinli
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Protein complexes are a cornerstone of many biological processes and together they form various types of molecular machinery.
A broad understanding of these protein complexes is crucial for revealing and building models of protein function and regulation.
Pancreatic cancer is a highly lethal disease which is difficult to diagnose at early stage and even more difficult to cure.
In this study, we applied a gradient clear native gel system combined with subsequent second-dimensional SDS-PAGE to separate protein complexes from cell lysates of SW1990 and PANC-1 pancreatic cancer cell lines with different degrees of differentiation.
Ten heat-shock-protein- (HSP-) associated protein complexes were separated and identified, and the differentially expressed proteins related to cancers were also found, such as HSP60, protein disulfide-isomerase A4 (ERp72), and transitional endoplasmic reticulum ATPase (TER ATPase).
American Psychological Association (APA)
Zhao, Zhiyun& Liu, Hui& Wang, Xinli& Wang, Xiaodong& Li, Zhili. 2011. Separation and Identification of HSP-Associated Protein Complexes from Pancreatic Cancer Cell Lines Using 2D CNSDS-PAGE Coupled with Mass Spectrometry. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-453381
Modern Language Association (MLA)
Zhao, Zhiyun…[et al.]. Separation and Identification of HSP-Associated Protein Complexes from Pancreatic Cancer Cell Lines Using 2D CNSDS-PAGE Coupled with Mass Spectrometry. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-453381
American Medical Association (AMA)
Zhao, Zhiyun& Liu, Hui& Wang, Xinli& Wang, Xiaodong& Li, Zhili. Separation and Identification of HSP-Associated Protein Complexes from Pancreatic Cancer Cell Lines Using 2D CNSDS-PAGE Coupled with Mass Spectrometry. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-453381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453381