Low-Frequency Low-Intensity Ultrasounds Do Not Influence the Survival and Immune Functions of Cultured Keratinocytes and Dendritic Cells
Joint Authors
De Pità, Ornella
Sestito, Rosanna
Pavani, Francesca
Albanesi, Cristina
Simonacci, Marco
Madonna, Stefania
Cavani, Andrea
Nasorri, Francesca
Scarponi, Claudia
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Low-frequency ultrasounds (US) are used to enhance drug transdermal transport.
Although this phenomenon has been extensively analyzed, information on US effects on the single skin cell components is limited.
Here, we investigated the possible effects of low-frequency US on viability and immune functions of cultured human keratinocytes and dendritic cells (DC), skin cells involved in the regulation of many immune-mediated dermatoses.
We demonstrated that US, employed at low-frequency (42 KHz) and low-intensity (0.15 W/cm2) values known to enhance drug and water transdermal transport, did not affect extracellular-signal-regulated-kinase (ERK)1/2 activation, cell viability, or expression of adhesion molecules in cultured keratinocytes.
Moreover, US at these work frequency and intensity did not influence the keratinocyte expression and release of immunomodulatory molecules.
Similarly, cultured DC treated with low-frequency low-intensity US were viable, and did not show an altered membrane phenotype, cytokine profile, nor antigen presentation ability.
However, intensity enhancement of low-frequency US to 5 W/cm2 determined an increase of the apoptotic rate of both keratinocytes and DC as well as keratinocyte CXCL8 release and ERK1/2 activation, and DC CD40 expression.
Our study sustains the employment of low-frequency and low-intensity US for treatment of those immune skin disorders, where keratinocytes and DC have a pathogenetic role.
American Psychological Association (APA)
Scarponi, Claudia& Nasorri, Francesca& Pavani, Francesca& Madonna, Stefania& Sestito, Rosanna& Simonacci, Marco…[et al.]. 2010. Low-Frequency Low-Intensity Ultrasounds Do Not Influence the Survival and Immune Functions of Cultured Keratinocytes and Dendritic Cells. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-453410
Modern Language Association (MLA)
Scarponi, Claudia…[et al.]. Low-Frequency Low-Intensity Ultrasounds Do Not Influence the Survival and Immune Functions of Cultured Keratinocytes and Dendritic Cells. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-12.
https://search.emarefa.net/detail/BIM-453410
American Medical Association (AMA)
Scarponi, Claudia& Nasorri, Francesca& Pavani, Francesca& Madonna, Stefania& Sestito, Rosanna& Simonacci, Marco…[et al.]. Low-Frequency Low-Intensity Ultrasounds Do Not Influence the Survival and Immune Functions of Cultured Keratinocytes and Dendritic Cells. Journal of Biomedicine and Biotechnology. 2010. Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-453410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453410