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Panax Quinquefolius Saponin of Stem and Leaf Attenuates Intermittent High Glucose-Induced Oxidative Stress Injury in Cultured Human Umbilical Vein Endothelial Cells via PI3KAktGSK-3β Pathway
Joint Authors
Zhang, Lu
Guo, Chunyu
Xia, Chengdong
Yin, Hui-jun
Liu, Qian
Wang, Jing-Shang
Huang, Ye
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Panax quinquefolius saponin of stem and leaf (PQS), the effective parts of American ginseng, is widely used in China as a folk medicine for diabetes and cardiovascular diseases treatment.
In our previous studies, we have demonstrated that PQS could improve the endothelial function of type II diabetes mellitus (T2DM) rats with high glucose fluctuation.
In the present study, we investigated the protective effects of PQS against intermittent high glucose-induced oxidative damage on human umbilical vein endothelial cells (HUVECs) and the role of phosphatidylinositol 3-kinase kinase (PI3K)/Akt/GSK-3β pathway involved.
Our results suggested that exposure of HUVECs to a high glucose concentration for 8 days showed a great decrease in cell viability accompanied by marked MDA content increase and SOD activity decrease.
Moreover, high glucose significantly reduced the phosphorylation of Akt and GSK-3β.
More importantly, these effects were even more evident in intermittent high glucose condition.
PQS treatment significantly attenuated intermittent high glucose-induced oxidative damage on HUVECs and meanwhile increased cell viability and phosphorylation of Akt and GSK-3β of HUVECs.
Interestingly, all these reverse effects of PQS on intermittent high glucose-cultured HUVECs were inhibited by PI3K inhibitor LY294002.
These findings suggest that PQS attenuates intermittent-high-glucose-induced oxidative stress injury in HUVECs by PI3K/Akt/GSK-3β pathway.
American Psychological Association (APA)
Wang, Jing-Shang& Yin, Hui-jun& Huang, Ye& Guo, Chunyu& Xia, Chengdong& Liu, Qian…[et al.]. 2013. Panax Quinquefolius Saponin of Stem and Leaf Attenuates Intermittent High Glucose-Induced Oxidative Stress Injury in Cultured Human Umbilical Vein Endothelial Cells via PI3KAktGSK-3β Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-453680
Modern Language Association (MLA)
Wang, Jing-Shang…[et al.]. Panax Quinquefolius Saponin of Stem and Leaf Attenuates Intermittent High Glucose-Induced Oxidative Stress Injury in Cultured Human Umbilical Vein Endothelial Cells via PI3KAktGSK-3β Pathway. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-453680
American Medical Association (AMA)
Wang, Jing-Shang& Yin, Hui-jun& Huang, Ye& Guo, Chunyu& Xia, Chengdong& Liu, Qian…[et al.]. Panax Quinquefolius Saponin of Stem and Leaf Attenuates Intermittent High Glucose-Induced Oxidative Stress Injury in Cultured Human Umbilical Vein Endothelial Cells via PI3KAktGSK-3β Pathway. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-453680
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453680