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High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
Joint Authors
Biegańska, Malgorzata
Dworecka-Kaszak, Bozena
Szopa, Daniel
Krutkiewicz, Alicja
Kleczkowski, Miroslaw
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-24
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents.
This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows.
Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated.
Seven different species classified as Candida were identified basing on phenotypic properties, and the dominating species was C.
parapsilosis.
Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers.
Amplification products were digested with restriction enzymes HpaII and EcoRI.
Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes.
Comparison of product sizes in restriction fragment PCR REA confirmed differences among species.
Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles.
None of the investigated species showed similar genetic profiles.
American Psychological Association (APA)
Dworecka-Kaszak, Bozena& Krutkiewicz, Alicja& Szopa, Daniel& Kleczkowski, Miroslaw& Biegańska, Malgorzata. 2012. High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-453685
Modern Language Association (MLA)
Dworecka-Kaszak, Bozena…[et al.]. High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland. The Scientific World Journal No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-453685
American Medical Association (AMA)
Dworecka-Kaszak, Bozena& Krutkiewicz, Alicja& Szopa, Daniel& Kleczkowski, Miroslaw& Biegańska, Malgorzata. High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-453685
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453685