In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells
Joint Authors
Omran, Moataza Hassan
El Desouki, Mohamed Ali
Fahmy, Ahmed Mohamed
Mohamed, Amr Saad
El-Awady, Mostafa K.
Youssef, Samar Samir
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Aim.
To assess the efficiency of phosphorothioate antisense oligodeoxynucleotide 1 (S-ODN1) on HCV translation inhibition in PBMC compared to hepatoma cells in vitro for the first time.
Materials and Methods.
The study included 34 treatment naive HCV patients.
IRES domain III and IV sequence variations were tested in 45 clones from 9 HCV patients.
PBMC of HCV positive patients were subjected to S-ODN in vitro.
Concomitantly HepG2 cells infected by the same patient’s serum were also treated with S-ODN1 for 24 and 48 hours.
Cellular RNA was tested for HCV plus and minus strands by reverse transcription polymerase chain reaction (RT-PCR).
Results.
Sequence variations were seen in HCV IRES domain III only while domain IV was conserved among all the tested patient’s clones.
S-ODN1 successfully inhibited HCV translation in HepG2 cells, while in PBMC inhibition was partial.
Conclusion.
HCV IRES domain IV is more conserved than domain IIId in genotype 4 HCV patients.
S-ODN against HCV IRES domain IV was not efficient to inhibit HCV translation in PBMC under the study conditions.
Further studies testing other S-ODN targeting other HCV IRES domains in PBMC should be done.
American Psychological Association (APA)
Youssef, Samar Samir& Fahmy, Ahmed Mohamed& Omran, Moataza Hassan& Mohamed, Amr Saad& El Desouki, Mohamed Ali& El-Awady, Mostafa K.. 2014. In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-453722
Modern Language Association (MLA)
Youssef, Samar Samir…[et al.]. In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-453722
American Medical Association (AMA)
Youssef, Samar Samir& Fahmy, Ahmed Mohamed& Omran, Moataza Hassan& Mohamed, Amr Saad& El Desouki, Mohamed Ali& El-Awady, Mostafa K.. In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-453722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453722