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Reliable Assessment and Quantification of the Fluorescence-Labeled Antisense Oligonucleotides In Vivo
Joint Authors
Chiara Munisso, Maria
Yamaoka, Tetsuji
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The availability of fluorescent dyes and the advances in the optical systems for in vivo imaging have stimulated an increasing interest in developing new methodologies to study and quantify the biodistribution of labeled agents.
However, despite these great achievements, we are facing significant challenges in determining if the observed fluorescence does correspond to the quantity of the dye in the tissues.
In fact, although the far-red and near-infrared lights can propagate through several centimetres of tissue, they diffuse within a few millimetres as consequence of the elastic scattering of photons.
In addition, when dye-labeled oligonucleotides form stable complex with cationic carriers, a large change in the fluorescence intensity of the dye is observed.
Therefore, the measured fluorescence intensity is altered by the tissue heterogeneity and by the fluctuation of dye intensity.
Hence, in this study a quantification strategy for fluorescence-labeled oligonucleotides was developed to solve these disadvantageous effects.
Our results proved that upon efficient homogenization and dilution with chaotropic agents, such as guanidinium thiocyanate, it is possible to achieve a complete fluorescence intensity recovery.
Furthermore, we demonstrated that this method has the advantage of good sensitivity and reproducibility, as well as easy handling of the tissue samples.
American Psychological Association (APA)
Chiara Munisso, Maria& Yamaoka, Tetsuji. 2014. Reliable Assessment and Quantification of the Fluorescence-Labeled Antisense Oligonucleotides In Vivo. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-453733
Modern Language Association (MLA)
Chiara Munisso, Maria& Yamaoka, Tetsuji. Reliable Assessment and Quantification of the Fluorescence-Labeled Antisense Oligonucleotides In Vivo. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-453733
American Medical Association (AMA)
Chiara Munisso, Maria& Yamaoka, Tetsuji. Reliable Assessment and Quantification of the Fluorescence-Labeled Antisense Oligonucleotides In Vivo. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-453733
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453733