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Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays
Joint Authors
Varanda, Leandro L.
Leitão, Alvaro C.
Caldeira-de-Araújo, Adriano
Motta, Ellen S.
Silva, Claudia R. da
Almeida, Gabriella S. de
Oliveira, Marcia B. N.
Pádula, Marcelo de
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Papain, a phytotherapeutic agent, has been used in the treatment of eschars and as a debriding chemical agent to remove damaged or necrotic tissue of pressure ulcers and gangrene.
Its benefits in these treatments are deemed effective, since more than 5000 patients, at the public university hospital at Rio de Janeiro, Brazil, have undergone papain treatment and presented satisfactory results.
Despite its extensive use, there is little information about toxic and mutagenic properties of papain.
This work evaluated the toxic and mutagenic potential of papain and its potential antioxidant activity against induced-H2O2 oxidative stress in Escherichia coli strains.
Cytotoxicity assay, Growth inhibition test, WP2-Mutoxitest and Plasmid-DNA treatment, and agarose gel electrophoresis were used to investigate if papain would present any toxic or mutagenic potential as well as if papain would display antioxidant properties.
Papain exhibited negative results for all tests.
This agent presented an activity protecting cells against H2O2-induced mutagenesis.
American Psychological Association (APA)
Silva, Claudia R. da& Oliveira, Marcia B. N.& Motta, Ellen S.& Almeida, Gabriella S. de& Varanda, Leandro L.& Pádula, Marcelo de…[et al.]. 2010. Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-453833
Modern Language Association (MLA)
Silva, Claudia R. da…[et al.]. Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-453833
American Medical Association (AMA)
Silva, Claudia R. da& Oliveira, Marcia B. N.& Motta, Ellen S.& Almeida, Gabriella S. de& Varanda, Leandro L.& Pádula, Marcelo de…[et al.]. Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-453833
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453833