Comparison of LC-MS Assay and HPLC Assay of Busulfan in Clinical Pharmacokinetics Studies
Joint Authors
Gounder, Murugesan K.
Goodin, Susan
DiPaola, Robert S.
Lin, Hongxia
Strair, Roger K.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Busulfan is used in preparative regimens for bone marrow transplantation and timely busulfan plasma concentration reporting is critical for subsequent dose adjustment.
We compared two sensitive methods for pharmacokinetics studies including LC-MS assay and HPLC precolumn derivatization assay.
Chromatographic separation was performed on a Gemini C18 column.
Liquid-liquid extraction with ethyl acetate was used for plasma sample preparation.
Busulfan and internal standard ([2H8]-busulfan) were detected as ammonium adducts at m/z 264.2 and 272.2 for LC-MS assay.
For HPLC assay, the extraction from plasma was derivatized with 2-naphathalenethiol using synthesized internal standard (1,6-(methanesulfonyloxy)octane).
The Ex and Em wavelength was 255 nm and 370 nm.
The limit of detection was 15.6 ng/mL and 7.8 ng/mL for HPLC and LC-MS assay and good linearity ranging from 31.25–1000 ng/mL for HPLC and 15.6-1000 ng/mL for LC-MS assay.
The intra and interday assay precision were less than 9.2% and 12.0% for LC-MS and HPLC assay.
The pharmacokinetic parameters were estimated using noncompartmental pharmacokinetic model with WinNonlin.
Busulfan AUClast showed an average difference of 0.7% between the two methods.
The LC-MS method is accurate, reproducible, and requires less specimen, sample preparation and analysis time over the HPLC assay, making busulfan monitoring faster and easier in clinical practice.
American Psychological Association (APA)
Lin, Hongxia& Goodin, Susan& Strair, Roger K.& DiPaola, Robert S.& Gounder, Murugesan K.. 2012. Comparison of LC-MS Assay and HPLC Assay of Busulfan in Clinical Pharmacokinetics Studies. ISRN Analytical Chemistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-453917
Modern Language Association (MLA)
Lin, Hongxia…[et al.]. Comparison of LC-MS Assay and HPLC Assay of Busulfan in Clinical Pharmacokinetics Studies. ISRN Analytical Chemistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-453917
American Medical Association (AMA)
Lin, Hongxia& Goodin, Susan& Strair, Roger K.& DiPaola, Robert S.& Gounder, Murugesan K.. Comparison of LC-MS Assay and HPLC Assay of Busulfan in Clinical Pharmacokinetics Studies. ISRN Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-453917
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453917