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IDH1R132H Mutation Increases U87 Glioma Cell Sensitivity to Radiation Therapy in Hypoxia
Joint Authors
Guillamo, Jean-Sébastien
Labussière, Marianne
Bernaudin, Myriam
Wang, Xiao-Wei
Pérès, Elodie A.
Sanson, Marc
Valable, Samuel
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Objective.
IDH1 codon 132 mutation (mostly Arg132His) is frequently found in gliomas and is associated with longer survival.
However, it is still unclear whether IDH1 mutation renders the cell more vulnerable to current treatment, radio- and chemotherapy.
Materials and Methods.
We transduced U87 with wild type IDH1 or IDH1R132H expressing lentivirus and analyzed the radiosensitivity (dose ranging 0 to 10 Gy) under normoxia (20% O2) and moderate hypoxia (1% O2).
Results.
We observed that IDH1R132H U87 cells grow faster in hypoxia and were more sensitive to radiotherapy (in terms of cell mortality and colony formation assay) compared to nontransduced U87 and IDH1wt cells.
This effect was not observed in normoxia.
Conclusion.
These data suggest that IDH1R132H mutation increases radiosensitivity in mild hypoxic conditions.
American Psychological Association (APA)
Wang, Xiao-Wei& Labussière, Marianne& Valable, Samuel& Pérès, Elodie A.& Guillamo, Jean-Sébastien& Bernaudin, Myriam…[et al.]. 2014. IDH1R132H Mutation Increases U87 Glioma Cell Sensitivity to Radiation Therapy in Hypoxia. BioMed Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-453920
Modern Language Association (MLA)
Wang, Xiao-Wei…[et al.]. IDH1R132H Mutation Increases U87 Glioma Cell Sensitivity to Radiation Therapy in Hypoxia. BioMed Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-453920
American Medical Association (AMA)
Wang, Xiao-Wei& Labussière, Marianne& Valable, Samuel& Pérès, Elodie A.& Guillamo, Jean-Sébastien& Bernaudin, Myriam…[et al.]. IDH1R132H Mutation Increases U87 Glioma Cell Sensitivity to Radiation Therapy in Hypoxia. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-453920
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453920