Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine
Joint Authors
Hajj Chehade, M.
Merlin, C.
Guilloteau, H.
Phe, M. H.
Block, J. C.
Source
International Journal of Microbiology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-07-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Water disinfection is usually evaluated using mandatory methods based on cell culturability.
However, such methods do not consider the potential of cells to recover, which should also be kept as low as possible.
In this paper, we hypothesized that a successful disinfection is achieved only when the applied chlorine leads to both intracellular nucleic acid damage and strong alterations of the DNA repair machinery.
Monitoring the SOS system responsiveness with a umuC'-‘lacZ reporter fusion, we found that the expression of this important cellular machinery was altered after the beginning of membrane permeabilization but prior to the total decline of both the cell culturability and the nucleic acid integrity as revealed by Sybr-II staining.
Rapid measurement of such nucleic acid alterations by fluorochrome-based staining could be used as an alternative method for assessing the effectiveness of disinfection with chlorine.
American Psychological Association (APA)
Phe, M. H.& Hajj Chehade, M.& Guilloteau, H.& Merlin, C.& Block, J. C.. 2009. Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine. International Journal of Microbiology،Vol. 2009, no. 2009, pp.1-5.
https://search.emarefa.net/detail/BIM-454039
Modern Language Association (MLA)
Phe, M. H.…[et al.]. Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine. International Journal of Microbiology No. 2009 (2009), pp.1-5.
https://search.emarefa.net/detail/BIM-454039
American Medical Association (AMA)
Phe, M. H.& Hajj Chehade, M.& Guilloteau, H.& Merlin, C.& Block, J. C.. Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine. International Journal of Microbiology. 2009. Vol. 2009, no. 2009, pp.1-5.
https://search.emarefa.net/detail/BIM-454039
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454039