Cell Uptake and Validation of Novel PECs for Biomedical Applications
Joint Authors
D'Amone, Stefania
Coluccia, Addolorata M. L.
Palamà, Ilaria E.
Gigli, Giuseppe
Musarò, Mariarosaria
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This pilot study provides the proof of principle for biomedical application of novel polyelectrolyte complexes (PECs) obtained via electrostatic interactions between dextran sulphate (DXS) and poly(allylamine hydrochloride) (PAH).
Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that DXS/PAH polyelectrolyte complexes were Monodispersed with regular rounded-shape features and average diameters of 250 nm at 2 : 1 weight ratios of DXS/PAH.
Fluorescently labelled DXS and fluorescein-isothiocyanate- (FITC-)conjugate DXS were used to follow cell uptake efficiency of PECs and biodegradability of their enzymatically degradable DXS-layers by using confocal laser scanning microscopy (CLSM).
Moreover, quantitative MTT and Trypan Blue assays were employed to validate PECs as feasible and safe nanoscaled carriers at single-cell level without adverse effects on metabolism and viability.
American Psychological Association (APA)
Palamà, Ilaria E.& Musarò, Mariarosaria& Coluccia, Addolorata M. L.& D'Amone, Stefania& Gigli, Giuseppe. 2011. Cell Uptake and Validation of Novel PECs for Biomedical Applications. Journal of Drug Delivery،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-454180
Modern Language Association (MLA)
Palamà, Ilaria E.…[et al.]. Cell Uptake and Validation of Novel PECs for Biomedical Applications. Journal of Drug Delivery No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-454180
American Medical Association (AMA)
Palamà, Ilaria E.& Musarò, Mariarosaria& Coluccia, Addolorata M. L.& D'Amone, Stefania& Gigli, Giuseppe. Cell Uptake and Validation of Novel PECs for Biomedical Applications. Journal of Drug Delivery. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-454180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454180