Local Gene Delivery System by Bubble Liposomes and Ultrasound Exposure into Joint Synovium
Joint Authors
Yamamoto, Matsuo
Aramaki, Yukihiko
Negishi, Yoichi
Maruyama, Kazuo
Suzuki, Ryo
Tsunoda, Yuka
Oda, Yusuke
Endo-Takahashi, Yoko
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Recently, we have developed novel polyethylene glycol modified liposomes (bubble liposomes; BL) entrapping an ultrasound (US) imaging gas, which can work as a gene delivery tool with US exposure.
In this study, we investigated the usefulness of US-mediated gene transfer systems with BL into synoviocytes in vitro and joint synovium in vivo.
Highly efficient gene transfer could be achieved in the cultured primary synoviocytes transfected with the combination of BL and US exposure, compared to treatment with plasmid DNA (pDNA) alone, pDNA plus BL, or pDNA plus US.
When BL was injected into the knee joints of mice, and US exposure was applied transcutaneously to the injection site, highly efficient gene expression could be observed in the knee joint transfected with the combination of BL and US exposure, compared to treatment with pDNA alone, pDNA plus BL, or pDNA plus US.
The localized and prolonged gene expression was also shown by an in vivo luciferase imaging system.
Thus, this local gene delivery system into joint synovium using the combination of BL and US exposure may be an effective means for gene therapy in joint disorders.
American Psychological Association (APA)
Negishi, Yoichi& Tsunoda, Yuka& Endo-Takahashi, Yoko& Oda, Yusuke& Suzuki, Ryo& Maruyama, Kazuo…[et al.]. 2011. Local Gene Delivery System by Bubble Liposomes and Ultrasound Exposure into Joint Synovium. Journal of Drug Delivery،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-454210
Modern Language Association (MLA)
Negishi, Yoichi…[et al.]. Local Gene Delivery System by Bubble Liposomes and Ultrasound Exposure into Joint Synovium. Journal of Drug Delivery No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-454210
American Medical Association (AMA)
Negishi, Yoichi& Tsunoda, Yuka& Endo-Takahashi, Yoko& Oda, Yusuke& Suzuki, Ryo& Maruyama, Kazuo…[et al.]. Local Gene Delivery System by Bubble Liposomes and Ultrasound Exposure into Joint Synovium. Journal of Drug Delivery. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-454210
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454210