Genomic Profiling of a Human Leukemic Monocytic Cell-Line (THP-1)‎ Exposed to Alpha Particle Radiation

Abstract EN

This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool.

THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy.

Twenty-four hours and three days after exposure gene expression was monitored using microarray technology.

A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure.

Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure.

Among these genes, 6 genes were time and dose responsive and validated further using alternate technology.

These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling/communication.

This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative of α-particle exposure.