DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol
Joint Authors
Sahu, Sunil Kumar
Kandasamy, Kathiresan
Thangaraj, Muthusamy
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Mangroves and salt marsh species are known to synthesize a wide spectrum of polysaccharides and polyphenols including flavonoids and other secondary metabolites which interfere with the extraction of pure genomic DNA.
Although a plethora of plant DNA isolation protocols exist, extracting DNA from mangroves and salt marsh species is a challenging task.
This study describes a rapid and reliable cetyl trimethylammonium bromide (CTAB) protocol suited specifically for extracting DNA from plants which are rich in polysaccharides and secondary metabolites, and the protocol also excludes the use of expensive liquid nitrogen and toxic phenols.
Purity of extracted DNA was excellent as evident by A260/A280 ratio ranging from 1.78 to 1.84 and A260/A230 ratio was >2, which also suggested that the preparations were sufficiently free of proteins and polyphenolics/polysaccharide compounds.
DNA concentration ranged from 8.8 to 9.9 μg μL−1.
The extracted DNA was amenable to RAPD, restriction digestion, and PCR amplification of plant barcode genes (matK and rbcl).
The optimized method is suitable for both dry and fresh leaves.
The success of this method in obtaining high-quality genomic DNA demonstrated the broad applicability of this method.
American Psychological Association (APA)
Sahu, Sunil Kumar& Thangaraj, Muthusamy& Kandasamy, Kathiresan. 2012. DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol. ISRN Molecular Biology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-454309
Modern Language Association (MLA)
Sahu, Sunil Kumar…[et al.]. DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol. ISRN Molecular Biology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-454309
American Medical Association (AMA)
Sahu, Sunil Kumar& Thangaraj, Muthusamy& Kandasamy, Kathiresan. DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol. ISRN Molecular Biology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-454309
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454309