Rapid Screening for Flavone C-Glycosides in the Leaves of Different Species of Bamboo and Simultaneous Quantitation of Four Marker Compounds by HPLC-UVDAD
Joint Authors
Wang, Jin
Yue, Yong-de
Jiang, Hao
Tang, Feng
Source
International Journal of Analytical Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A strategy for analyzing flavone C-glucosides in the leaves of different species of bamboo was developed.
Firstly, the flavone C-glycosides were extracted from the bamboo leaves (51 species in 17 genera) with methanol and chromatographed on silica gel 60 plates in automatic developing chamber (ADC2), and a qualitative survey using simple derivatization steps with the NP reagent was carried out.
The flavone C-glycosides were found in 40 of 51 species of bamboo examined.
Secondly, an HPLC method with photodiode array and multiple wavelength detector was optimized and validated for the simultaneous determination of flavone C-glycosides, including isoorientin, isovitexin, orientin, and vitexin in the leaves of three species of bamboo and the flavone C-glycosides were confirmed by LC/MS.
The optimized HPLC method proved to be linear in the concentration range tested (0.2–100 μg/mL, r2≥0.9997), precise (RSD≤1.56%), and accurate (88–106%).
The concentration ranges of isoorientin, isovitexin, orientin, and vitexin in three bamboo leaves samples were 1.00–2.78, 0–0.31, 0–0.07, and 0.20–0.68 mg/g, respectively.
The proposed method was validated to be simple and reliable and can be a tool for quality control of bamboo leaf extract or its commercial products.
American Psychological Association (APA)
Wang, Jin& Yue, Yong-de& Jiang, Hao& Tang, Feng. 2012. Rapid Screening for Flavone C-Glycosides in the Leaves of Different Species of Bamboo and Simultaneous Quantitation of Four Marker Compounds by HPLC-UVDAD. International Journal of Analytical Chemistry،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-454313
Modern Language Association (MLA)
Wang, Jin…[et al.]. Rapid Screening for Flavone C-Glycosides in the Leaves of Different Species of Bamboo and Simultaneous Quantitation of Four Marker Compounds by HPLC-UVDAD. International Journal of Analytical Chemistry No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-454313
American Medical Association (AMA)
Wang, Jin& Yue, Yong-de& Jiang, Hao& Tang, Feng. Rapid Screening for Flavone C-Glycosides in the Leaves of Different Species of Bamboo and Simultaneous Quantitation of Four Marker Compounds by HPLC-UVDAD. International Journal of Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-454313
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454313