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The OPFOS Microscopy Family : High-Resolution Optical Sectioning of Biomedical Specimens
Joint Authors
Buytaert, Jan A. N.
Descamps, Emilie
Dirckx, Joris J. J.
Adriaens, Dominique
Source
Anatomy Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM).
The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light.
Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera.
In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions—orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)—is discussed.
Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss.
All these optical sectioning methods create images showing histological detail.
We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.
American Psychological Association (APA)
Buytaert, Jan A. N.& Descamps, Emilie& Adriaens, Dominique& Dirckx, Joris J. J.. 2011. The OPFOS Microscopy Family : High-Resolution Optical Sectioning of Biomedical Specimens. Anatomy Research International،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-454423
Modern Language Association (MLA)
Buytaert, Jan A. N.…[et al.]. The OPFOS Microscopy Family : High-Resolution Optical Sectioning of Biomedical Specimens. Anatomy Research International No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-454423
American Medical Association (AMA)
Buytaert, Jan A. N.& Descamps, Emilie& Adriaens, Dominique& Dirckx, Joris J. J.. The OPFOS Microscopy Family : High-Resolution Optical Sectioning of Biomedical Specimens. Anatomy Research International. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-454423
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454423