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Interaction of Helicobacter pylori with C-Type Lectin Dendritic Cell-Specific ICAM Grabbing Nonintegrin
Joint Authors
Chmiela, Magdalena
Walencka, Maria
Rudnicka, Wiesława
Kowalewicz-Kulbat, Magdalena
Druszczyńska, Magdalena
Fol, Marek
Matusiak, Agnieszka
Rudnicka, Karolina
Moran, Anthony P.
Miszczyk, Eliza
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In this study we asked whether Helicobacter pylori whole cells and lipopolysaccharide (LPS) utilize sugar moieties of Lewis (Le) antigenic determinants to interact with DC-SIGN (dendritic cell specific ICAM grabbing nonintegrin) receptor on dendritic cells (DCs).
For this purpose the soluble DC-SIGN/Fc adhesion assay and the THP-1 leukemia cells with induced expression of DC-SIGN were used.
We showed that the binding specificity of DC-SIGN with H.
pylori LeX/Y positive whole cells and H.
pylori LPS of LeX/Y type was fucose dependent, whereas in LeXY negative H.
pylori strains and LPS preparations without Lewis determinants, this binding was galactose dependent.
The binding of soluble synthetic LeX and LeY to the DC-SIGN-like receptor on THP-1 cells was also observed.
In conclusion, the LeXY dependent as well as independent binding of H.
pylori whole cells and H.
pylori LPS to DC-SIGN was described.
Moreover, we demonstrated that THP-1 cells may serve as an in vitro model for the assessment of H.
pylori-DC-SIGN interactions mediated by LeX and LeY determinants.
American Psychological Association (APA)
Miszczyk, Eliza& Rudnicka, Karolina& Moran, Anthony P.& Fol, Marek& Kowalewicz-Kulbat, Magdalena& Druszczyńska, Magdalena…[et al.]. 2012. Interaction of Helicobacter pylori with C-Type Lectin Dendritic Cell-Specific ICAM Grabbing Nonintegrin. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-454457
Modern Language Association (MLA)
Miszczyk, Eliza…[et al.]. Interaction of Helicobacter pylori with C-Type Lectin Dendritic Cell-Specific ICAM Grabbing Nonintegrin. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-454457
American Medical Association (AMA)
Miszczyk, Eliza& Rudnicka, Karolina& Moran, Anthony P.& Fol, Marek& Kowalewicz-Kulbat, Magdalena& Druszczyńska, Magdalena…[et al.]. Interaction of Helicobacter pylori with C-Type Lectin Dendritic Cell-Specific ICAM Grabbing Nonintegrin. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-454457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454457