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Time-Lapse Dynamics of the Mouse Oocyte Chromatin Organisation during Meiotic Resumption
Joint Authors
Vigone, Giulia
Zuccotti, Maurizio
Redi, Carlo Alberto
Merico, Valeria
Belli, Martina
Garagna, Silvia
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In the mammalian oocyte, distinct patterns of centromeres and pericentromeric heterochromatin localisation correlate with the gamete’s developmental competence.
Mouse antral oocytes display two main types of chromatin organisation: SN oocytes, with a ring of Hoechst-positive chromatin surrounding the nucleolus, and NSN oocytes lacking this ring.
When matured to MII and fertilised, only SN oocytes develop beyond the 2-cell, and reach full term.
To give detailed information on the dynamics of the SN or NSN chromatin during meiosis resumption, we performed a 9 hr time-lapse observation.
The main significant differences recorded are: (1) reduction of the nuclear area only in SN oocytes; (2) ~17 min delay of GVBD in NSN oocytes; (3) chromatin condensation, after GVBD, in SN oocytes; (4) formation of 4-5 CHCs in SN oocytes; (5) increase of the perivitelline space, ~57 min later in NSN oocytes; (6) formation of a rosette-like disposition of CHCs, ~84 min later in SN oocytes; (7) appearance of the MI plate ~40 min later in NSN oocytes.
Overall, we described a pathway of transition from the GV to the MII stage that is punctuated of discrete recordable events showing their specificity and occurring with different time kinetics in the two types of oocytes.
American Psychological Association (APA)
Belli, Martina& Vigone, Giulia& Merico, Valeria& Redi, Carlo Alberto& Garagna, Silvia& Zuccotti, Maurizio. 2014. Time-Lapse Dynamics of the Mouse Oocyte Chromatin Organisation during Meiotic Resumption. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-454545
Modern Language Association (MLA)
Belli, Martina…[et al.]. Time-Lapse Dynamics of the Mouse Oocyte Chromatin Organisation during Meiotic Resumption. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-454545
American Medical Association (AMA)
Belli, Martina& Vigone, Giulia& Merico, Valeria& Redi, Carlo Alberto& Garagna, Silvia& Zuccotti, Maurizio. Time-Lapse Dynamics of the Mouse Oocyte Chromatin Organisation during Meiotic Resumption. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-454545
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454545