Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan
Joint Authors
Badwi, Magdi
Sidahmed Abdelrahim, Egbal
Mohamed, Amal M.
Khalifa, Khalda A.
Source
Journal of Veterinary Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-18
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
The current study described the isolation and molecular detection of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae from tracheal swabs of diseased birds showing signs of respiratory distress in selected commercial (layer and broiler) farms and from yolk and an open air of pens of vaccinated breeder flocks in Sudan.
A number of 45 Mycoplasma isolates were recovered from chickens in Khartoum, Gezira, and Equatoria states in Sudan.
Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests.
The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively.
This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination.
PCR is considered a rapid, sensitive, and cheap method and it will improve the diagnosis of Mycoplasma in chickens.
American Psychological Association (APA)
Khalifa, Khalda A.& Sidahmed Abdelrahim, Egbal& Badwi, Magdi& Mohamed, Amal M.. 2013. Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan. Journal of Veterinary Medicine،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-454606
Modern Language Association (MLA)
Khalifa, Khalda A.…[et al.]. Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan. Journal of Veterinary Medicine No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-454606
American Medical Association (AMA)
Khalifa, Khalda A.& Sidahmed Abdelrahim, Egbal& Badwi, Magdi& Mohamed, Amal M.. Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan. Journal of Veterinary Medicine. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-454606
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454606