RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals
Joint Authors
Tamboli, Sagar R.
Patil, Dipak D.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A precise, accurate, sensitive and robust RP-HPLC method was developed for cefepime hydrochloride and tazobactam sodium in bulk and pharmaceutical formulation.
Chromatographic separation was achieved on PrincetonSPHER-100 C-18 column (250 mm × 4.6 mm i.d., 5 µm) at ambient temperature.
A binary mobile phase consisting of 25 mM potassium dihydrogen phosphate buffer, pH 6.2 and acetonitrile (94 : 6, v/v) was delivered through a column at a flow rate of 1 mL/min.
Measurement was performed at a wavelength 210 nm.
The method was linear over the concentration range of 4–24 µg/mL (r2=0.9977) for cefepime and 0.5–3.0 µg/mL (r2=0.9974) for tazobactam.
The percentage content found for cefepime was 101.12 ± 0.49 and for tazobactam was 101.33 ± 1.17 in the pharmaceutical formulation.
The method was validated for linearity, precision, accuracy, sensitivity and robustness as per ICH Q2 (R1) guideline.
American Psychological Association (APA)
Tamboli, Sagar R.& Patil, Dipak D.. 2013. RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-454609
Modern Language Association (MLA)
Tamboli, Sagar R.& Patil, Dipak D.. RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals. Journal of Chemistry No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-454609
American Medical Association (AMA)
Tamboli, Sagar R.& Patil, Dipak D.. RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-454609
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454609