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Instrument for Label-Free Detection of Noncoding RNAs
Joint Authors
Noy, Peter
Voelkle, Joerg
Fattinger, Christof
Hegner, Martin
Steiner, Roger
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-26
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
We set up a label-free direct binding assay for the detection of noncoding RNAs.
The assay is based on nanomechanical cantilever arrays for the detection of surface stress induced by immobilized biomolecules and their interaction partners.
We used various means to significantly reduce the drift of the cantilever readout that was a prominent feature in experiments with readout in stationary fluid before and after sample injection.
Major improvements were achieved by focusing on a faster system equilibration (for instance temperature control and diffusion independence).
Experimental protocols were improved to provide user-friendly and less time-consuming measurements.
Further enhancements were achieved by, for example, using pre-gold-coated cantilever array wafers compared to individually prepared ones and a directly implemented data analysis tool as real-time feature of the measurement software.
We have demonstrated picomolar specific biomarker target detection and can easily distinguish modified targets with single-nucleotide mismatches that hybridize with lower affinity.
American Psychological Association (APA)
Noy, Peter& Steiner, Roger& Voelkle, Joerg& Hegner, Martin& Fattinger, Christof. 2011. Instrument for Label-Free Detection of Noncoding RNAs. Journal of Sensors،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-454612
Modern Language Association (MLA)
Noy, Peter…[et al.]. Instrument for Label-Free Detection of Noncoding RNAs. Journal of Sensors No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-454612
American Medical Association (AMA)
Noy, Peter& Steiner, Roger& Voelkle, Joerg& Hegner, Martin& Fattinger, Christof. Instrument for Label-Free Detection of Noncoding RNAs. Journal of Sensors. 2011. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-454612
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454612