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Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
Joint Authors
Mogensen, Trine H.
Melchjorsen, Jesper
Søby, Stine
Zuwala, Kaja
Østergaard, Lars
Diget, Elisabeth A.
Laursen, Rune R.
Berg, Randi K.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Macrophages play an important role in human immunodeficiency virus (HIV) pathogenesis and contribute to establishment of a viral reservoir responsible for continuous virus production and virus transmission to T cells.
In this study, we investigated the differences between various monocyte-derived macrophages (MDMs) generated through different differentiation protocols and evaluated different cellular, immunological, and virological properties.
We found that elevated and persistent HIV-1 pWT/BaL replication could be obtained only in MDMs grown in RPMI containing macrophage colony-stimulating factor (M-CSF).
Interestingly, this MDM type was also most responsive to toll-like receptor stimulation.
By contrast, all MDM types were activated to a comparable extent by intracellular DNA, and the macrophage serum-free medium-(Mac-SFM-)differentiated MDMs responded strongly to membrane fusion through expression of CXCL10.
Finally, we found that HIV infection of RPMI/M-CSF-differentiated MDMs induced low-grade expression of two interferon-stimulated genes in some donors.
In conclusion, our study demonstrates that the differentiation protocol used greatly influences the ability of MDMs to activate innate immune reactions and support HIV-1 replication.
Paradoxically, the data show that the MDMs with the strongest innate immune response were also the most permissive for HIV-1 replication.
American Psychological Association (APA)
Diget, Elisabeth A.& Zuwala, Kaja& Berg, Randi K.& Laursen, Rune R.& Søby, Stine& Østergaard, Lars…[et al.]. 2013. Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-454646
Modern Language Association (MLA)
Diget, Elisabeth A.…[et al.]. Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages. Mediators of Inflammation No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-454646
American Medical Association (AMA)
Diget, Elisabeth A.& Zuwala, Kaja& Berg, Randi K.& Laursen, Rune R.& Søby, Stine& Østergaard, Lars…[et al.]. Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-454646
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454646