![](/images/graphics-bg.png)
Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
Joint Authors
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Sperm membranes change in structure and composition upon ejaculation to undergo capacitation, a molecular transformation which enables spermatozoa to undergo the acrosome reaction and be capable of fertilization.
Changes to the membrane environment including lipid composition, specifically lipid microdomains, may be responsible for enabling capacitation.
To study the effect of lipid environment on proteins, liposomes were created using lipids extracted from bull sperm membranes, with or without a protein (Na+ K+-ATPase or α-amylase).
Protein incorporation, function, and orientation were determined.
Fluorescence resonance energy transfer (FRET) confirmed protein inclusion in the lipid bilayer, and protein function was confirmed using a colourometric assay of phosphate production from ATP cleavage.
In the native lipid liposomes, ATPase was oriented with the β subunit facing the outer leaflet, while changing the lipid composition to 50% native lipids and 50% exogenous lipids significantly altered this orientation of Na+ K+-ATPase within the membranes.
American Psychological Association (APA)
Hickey, Katie D.& Buhr, Mary M.. 2011. Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function. Journal of Lipids،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-454652
Modern Language Association (MLA)
Hickey, Katie D.& Buhr, Mary M.. Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function. Journal of Lipids No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-454652
American Medical Association (AMA)
Hickey, Katie D.& Buhr, Mary M.. Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function. Journal of Lipids. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-454652
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454652