A Novel Platelet Concentrate : Titanium-Prepared Platelet-Rich Fibrin

Abstract EN

We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF).

The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun’s leukocyte- and platelet-rich fibrin (L-PRF) method.

In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF.

Blood samples were collected from 10 healthy male volunteers.

The blood samples were drawn using a syringe.

Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube.

Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF) was processed with a scanning electron microscope (SEM).

The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis.

The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples.

Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples.

This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product.

The platelet activation by titanium seems to offer some high characteristics to T-PRF.