5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice

Abstract EN

The expression of 5-lipoxygenase (5-LOX) is affected by aging and regulated by epigenetic mechanisms including DNA methylation.

We used methylation-sensitive restriction endonucleases (AciI, BstUI, HpaII, and HinP1I) to assess 5-LOX DNA methylation in brain and heart tissue samples from young (2 months) and old (22 months) mice.

We also measured mRNA content for 5-LOX and the DNA methyltransferases DNMT1 and DNMT3a.

In young mice, the 5-LOX mRNA content was significantly greater in the heart compared to the brain; 5-LOX DNA methylation was lower, except in the AciI assay in which it was higher in the heart.

Aging decreased 5-LOX mRNA content in the heart and increased it in the brain.

Aging also increased 5-LOX DNA methylation and this effect was site- (i.e., enzyme) and tissue-specific.

Generally, DNMT1 and DNMT3a mRNA content was lower in the brain regions compared to the heart; the only effect of aging was observed in the mRNA content of DNMT3a, which was decreased in the heart of old mice.

These results indicate a complex tissue-specific and aging-dependent interplay between the DNA methylation system and 5-LOX mRNA content.

Interpretation of this data must take into account that the tissue samples contained a mixture of various cell types.