Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique : A Czech Multicenter Experience
Joint Authors
Smetana, Jan
Zahradová, Lenka
Almasi, Martina
Frohlich, Jan
Kuglik, Petr
Kupska, Renata
Greslikova, Henrieta
Adam, Zdenek
Vallova, Vladimira
Hajek, Roman
Mikulasova, Aneta
Zaoralova, Romana
Pour, Ludek
Nemec, Pavel
Sandecka, Viera
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Characteristic recurrent copy number aberrations (CNAs) play a key role in multiple myeloma (MM) pathogenesis and have important prognostic significance for MM patients.
Array-based comparative genomic hybridization (aCGH) provides a powerful tool for genome-wide classification of CNAs and thus should be implemented into MM routine diagnostics.
We demonstrate the possibility of effective utilization of oligonucleotide-based aCGH in 91 MM patients.
Chromosomal aberrations associated with effect on the prognosis of MM were initially evaluated by I-FISH and were found in 93.4% (85/91).
Incidence of hyperdiploidy was 49.5% (45/91); del(13)(q14) was detected in 57.1% (52/91); gain(1)(q21) occurred in 58.2% (53/91); del(17)(p13) was observed in 15.4% (14/91); and t(4;14)(p16;q32) was found in 18.6% (16/86).
Genome-wide screening using Agilent 44K aCGH microarrays revealed copy number alterations in 100% (91/91).
Most common deletions were found at 13q (58.9%), 1p (39.6%), and 8p (31.1%), whereas gain of whole 1q was the most often duplicated region (50.6%).
Furthermore, frequent homozygous deletions of genes playing important role in myeloma biology such as TRAF3, BIRC1/BIRC2, RB1, or CDKN2C were observed.
Taken together, we demonstrated the utilization of aCGH technique in clinical diagnostics as powerful tool for identification of unbalanced genomic abnormalities with prognostic significance for MM patients.
American Psychological Association (APA)
Smetana, Jan& Frohlich, Jan& Zaoralova, Romana& Vallova, Vladimira& Greslikova, Henrieta& Kupska, Renata…[et al.]. 2014. Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique : A Czech Multicenter Experience. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-454774
Modern Language Association (MLA)
Smetana, Jan…[et al.]. Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique : A Czech Multicenter Experience. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-454774
American Medical Association (AMA)
Smetana, Jan& Frohlich, Jan& Zaoralova, Romana& Vallova, Vladimira& Greslikova, Henrieta& Kupska, Renata…[et al.]. Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique : A Czech Multicenter Experience. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-454774
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454774