Investigation of Effects and Mechanisms of Total Flavonoids of Astragalus and Calycosin on Human Erythroleukemia Cells
Joint Authors
Wang, Haibao
Pan, Jichun
Yu, Yang
Wang, Deqing
Zhang, Dongqing
Zhuang, Yuan
Li, Hui
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-27
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Flavonoids are found in most parts of plants and have been shown to have multiple biological activities such as anticancer, anti-inflammation, antibacteria, antivirus, and immune-stimulation.
Existing data showed that the total flavonoids of Astragalus (TFA) can provide biological system with resistance to injury and can possess antimutagenic, atherosclerotic inhibition, and other biological effects.
This study investigated the effects of TFA and calycosin (a compound isolated from TFA), on apoptosis induction, and cell cycle of human erythroleukemia cell line K562 by an array of techniques, including proliferation (MTT), PI staining, Annexin V/PI double staining, and RT-PCR.
The experimental data showed that TFA and calycosin could inhibit the proliferation of K562 cells.
The 50% inhibiting concentrations of TFA and calycosin were 98.63 μg/mL and 130.32 μg/mL, respectively.
However, TFA and calycosin could not induce apoptosis in K562 cells, but could increase the number of the cells in the G0/G1 phase.
The level of cyclin D1 mRNA in K562 cells decreased after the treatment with TFA and calycosin.
This study provides new insights into the functional mechanism of total flavonoids of Astragalus and calycosin on human erythroleukemia cells.
American Psychological Association (APA)
Zhang, Dongqing& Zhuang, Yuan& Pan, Jichun& Wang, Haibao& Li, Hui& Yu, Yang…[et al.]. 2012. Investigation of Effects and Mechanisms of Total Flavonoids of Astragalus and Calycosin on Human Erythroleukemia Cells. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-454798
Modern Language Association (MLA)
Zhang, Dongqing…[et al.]. Investigation of Effects and Mechanisms of Total Flavonoids of Astragalus and Calycosin on Human Erythroleukemia Cells. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-454798
American Medical Association (AMA)
Zhang, Dongqing& Zhuang, Yuan& Pan, Jichun& Wang, Haibao& Li, Hui& Yu, Yang…[et al.]. Investigation of Effects and Mechanisms of Total Flavonoids of Astragalus and Calycosin on Human Erythroleukemia Cells. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-454798
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454798