Expression and Significances of Contactin-1 in Human Gastric Cancer

Abstract EN

Background.

This study aimed at determining the relationship between vascular endothelial growth factor-C (VEGF-C), vascular endothelial growth factor receptor-3 (VEGFR-3), and contactin-1 (CNTN-1) expression in gastric cancer (GC).

Methods.

The expression level of CNTN-1 mRNA and CNTN-1 protein of 33 cases was determined using RT-PCR and Western Blot.

And 105 cases were immunohistochemically examined for VEGF-C, VEGFR-3, and CNTN-1 expressions.

Assessment of lymphatic vessel density (LVD) was also performed by D2-40 immunostaining.

Then we analyzed the relationships between VEGF-C, VEGFR-3, and CNTN-1, as well as their correlations with clinicopathologic features, LVD, and survival time.

Results.

The positivity rate of VEGF-C, VEGFR-3, and CNTN-1 in primary tumor was 56.19%, 64.76%, and 58.09%.

The expression of CNTN-1 significantly correlated with VEGF-C (P<0.001) and VEGFR-3 (P<0.001).

All of them were closely related to TNM stage, lymphatic invasion, and lymph node involvement (P<0.05).

LVD was significantly correlated with VEGF-C (P=0.001), VEGFR-3 (P=0.011), and CNTN-1 expression (P<0.001).

VEGF-C, VEGFR-3, and CNTN-1 expression significantly associated with poorer prognosis (P<0.001, P=0.034, P=0.012, resp.).

Conclusion.

CNTN-1 associated with VEGF-C and VEGFR-3 expression in GC.

All of them correlated with lymphatic metastasis, which might play an important role in the lymphatic invasion via lymphangiogenesis pathway in GC.