Explanting Is an Ex Vivo Model of Renal Epithelial-Mesenchymal Transition
Joint Authors
Kelynack, Kristen J.
Winbanks, Catherine E.
Hewitson, Tim D.
Becker, Gavin J.
Pouniotis, Dodie
Darby, Ian A.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Recognised by their de novo expression of alpha-smooth muscle actin (SMA), recruitment of myofibroblasts is key to the pathogenesis of fibrosis in chronic kidney disease.
Increasingly, we realise that epithelial-mesenchymal transition (EMT) may be an important source of these cells.
In this study we describe a novel model of renal EMT.
Rat kidney explants were finely diced on gelatin-coated Petri dishes and cultured in serum-supplemented media.
Morphology and immunocytochemistry were used to identify mesenchymal (vimentin+, α-smooth muscle actin (SMA)+, desmin+), epithelial (cytokeratin+), and endothelial (RECA+) cells at various time points.
Cell outgrowths were all epithelial in origin (cytokeratin+) at day 3.
By day 10, 50 ± 12% (mean ± SE) of cytokeratin+ cells double-labelled for SMA, indicating EMT.
Lectin staining established a proximal tubule origin.
By day 17, cultures consisted only of myofibroblasts (SMA+/cytokeratin−).
Explanting is a reproducible ex vivo model of EMT.
The ability to modify this change in phenotype provides a useful tool to study the regulation and mechanisms of renal tubulointerstitial fibrosis.
American Psychological Association (APA)
Winbanks, Catherine E.& Darby, Ian A.& Kelynack, Kristen J.& Pouniotis, Dodie& Becker, Gavin J.& Hewitson, Tim D.. 2011. Explanting Is an Ex Vivo Model of Renal Epithelial-Mesenchymal Transition. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-454963
Modern Language Association (MLA)
Winbanks, Catherine E.…[et al.]. Explanting Is an Ex Vivo Model of Renal Epithelial-Mesenchymal Transition. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-454963
American Medical Association (AMA)
Winbanks, Catherine E.& Darby, Ian A.& Kelynack, Kristen J.& Pouniotis, Dodie& Becker, Gavin J.& Hewitson, Tim D.. Explanting Is an Ex Vivo Model of Renal Epithelial-Mesenchymal Transition. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-454963
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-454963