Characterization of Zebrafish von Willebrand Factor Reveals Conservation of Domain Structure, Multimerization, and Intracellular Storage
Joint Authors
Vo, Andy
Kretz, Colin A.
Twiss, Beverly K.
Jozwiak, Mary A.
Montgomery, Robert R.
Shavit, Jordan A.
Ghosh, Arunima
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
von Willebrand disease (VWD) is the most common inherited human bleeding disorder and is caused by quantitative or qualitative defects in von Willebrand factor (VWF).
VWF is a secreted glycoprotein that circulates as large multimers.
While reduced VWF is associated with bleeding, elevations in overall level or multimer size are implicated in thrombosis.
The zebrafish is a powerful genetic model in which the hemostatic system is well conserved with mammals.
The ability of this organism to generate thousands of offspring and its optical transparency make it unique and complementary to mammalian models of hemostasis.
Previously, partial clones of zebrafish vwf have been identified, and some functional conservation has been demonstrated.
In this paper we clone the complete zebrafish vwf cDNA and show that there is conservation of domain structure.
Recombinant zebrafish Vwf forms large multimers and pseudo-Weibel-Palade bodies (WPBs) in cell culture.
Larval expression is in the pharyngeal arches, yolk sac, and intestinal epithelium.
These results provide a foundation for continued study of zebrafish Vwf that may further our understanding of the mechanisms of VWD.
American Psychological Association (APA)
Ghosh, Arunima& Vo, Andy& Twiss, Beverly K.& Kretz, Colin A.& Jozwiak, Mary A.& Montgomery, Robert R.…[et al.]. 2012. Characterization of Zebrafish von Willebrand Factor Reveals Conservation of Domain Structure, Multimerization, and Intracellular Storage. Advances in Hematology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-455096
Modern Language Association (MLA)
Ghosh, Arunima…[et al.]. Characterization of Zebrafish von Willebrand Factor Reveals Conservation of Domain Structure, Multimerization, and Intracellular Storage. Advances in Hematology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-455096
American Medical Association (AMA)
Ghosh, Arunima& Vo, Andy& Twiss, Beverly K.& Kretz, Colin A.& Jozwiak, Mary A.& Montgomery, Robert R.…[et al.]. Characterization of Zebrafish von Willebrand Factor Reveals Conservation of Domain Structure, Multimerization, and Intracellular Storage. Advances in Hematology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-455096
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455096