![](/images/graphics-bg.png)
Optimization of Molecularly Imprinted Polymer Method for Rapid Screening of 17β-Estradiol in Water by Fluorescence Quenching
Joint Authors
Source
International Journal of Analytical Chemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A new method was optimized for rapid screening of 17β-estradiol (E2) in water under 10 min.
Molecularly imprinted polymer (MIP) particles (325 ± 25 nm) were added in a water sample at pH 5.5 and 20∘C to form a suspension.
Fluorescence emission from E2 nonspecifically bound onto the MIP particles was first quenched by large gold nanoparticles (43 ± 5 nm).
The Stern-Volmer plot was linear, with dynamic quenching constants (Ksv) of 2.9 ×104 M-1.
Fluorescence emission from E2 specifically bound inside the MIP particles was next quenched by small nitrite anions that easily penetrated the imprinted cavities.
The Stern-Volmer plot became nonlinear, with Ksv = 2.1 × 102 M-1 and static quenching constant (V) below 1.0 M-1.
The difference between these two emission intensities varied as the initial E2 concentration in water, generating a Scatchard calibration curve with R2>0.97 from 0.1 to 10 ppb.
American Psychological Association (APA)
Yang, Yu& Lai, Edward P. C.. 2011. Optimization of Molecularly Imprinted Polymer Method for Rapid Screening of 17β-Estradiol in Water by Fluorescence Quenching. International Journal of Analytical Chemistry،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-455141
Modern Language Association (MLA)
Yang, Yu& Lai, Edward P. C.. Optimization of Molecularly Imprinted Polymer Method for Rapid Screening of 17β-Estradiol in Water by Fluorescence Quenching. International Journal of Analytical Chemistry No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-455141
American Medical Association (AMA)
Yang, Yu& Lai, Edward P. C.. Optimization of Molecularly Imprinted Polymer Method for Rapid Screening of 17β-Estradiol in Water by Fluorescence Quenching. International Journal of Analytical Chemistry. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-455141
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455141