Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic Microscopy Ex Vivo
Joint Authors
Wu, Hao
Wang, Cheng
Ping, Bo
Yang, Ling
Shi, Da-Ren
Liu, Wen-Tao
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-10
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Confocal light absorption and scattering spectroscopic (CLASS) microscopy can detect changes in biochemicals and the morphology of cells.
It is therefore used to detect high-grade cervical squamous intraepithelial lesion (HSIL) cells in the diagnosis of premalignant cervical lesions.
Forty cervical samples from women with abnormal Pap smear test results were collected, and twenty cases were diagnosed as HSIL; the rest were normal or low-grade cervical squamous intraepithelial lesion (LSIL).
The enlarged and condensed nuclei of HSIL cells as viewed under CLASS microscopy were much brighter and bigger than those of non-HSIL cells.
Cytological elastic scattered light data was then collected at wavelengths between 400 and 1000 nm.
Between 600 nm to 800 nm, the relative elastic scattered light intensity of HSIL cells was higher than that of the non-HSIL.
Relative intensity peaks occurred at 700 nm and 800 nm.
CLASS sensitivity and specificity results for HSIL and non-HSIL compared to cytology diagnoses were 80% and 90%, respectively.
This study demonstrated that CLASS microscopy could effectively detect cervical precancerous lesions.
Further study will verify this conclusion before the method is used in clinic for early detection of cervical cancer.
American Psychological Association (APA)
Yang, Ling& Liu, Wen-Tao& Wu, Hao& Wang, Cheng& Ping, Bo& Shi, Da-Ren. 2011. Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic Microscopy Ex Vivo. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-455147
Modern Language Association (MLA)
Yang, Ling…[et al.]. Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic Microscopy Ex Vivo. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-455147
American Medical Association (AMA)
Yang, Ling& Liu, Wen-Tao& Wu, Hao& Wang, Cheng& Ping, Bo& Shi, Da-Ren. Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic Microscopy Ex Vivo. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-455147
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455147