![](/images/graphics-bg.png)
Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal
Joint Authors
Zakaria, Zuki Abu Bakar
Shafiu Kamba, Abdullahi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Calcium carbonate (CaCO3) nanocrystals derived from cockle shells emerge to present a good concert in bone tissue engineering because of their potential to mimic the composition, structure, and properties of native bone.
The aim of this study was to evaluate the biological response of CaCO3 nanocrystals on hFOB 1.19 and MC3T3 E-1 osteoblast cells in vitro.
Cell viability and proliferation were assessed by MTT and BrdU assays, and LDH was measured to determine the effect of CaCO3 nanocrystals on cell membrane integrity.
Cellular morphology was examined by SEM and fluorescence microscopy.
The results showed that CaCO3 nanocrystals had no toxic effects to some extent.
Cell proliferation, alkaline phosphatase activity, and protein synthesis were enhanced by the nanocrystals when compared to the control.
Cellular interactions were improved, as indicated by SEM and fluorescent microscopy.
The production of VEGF and TGF-1 was also affected by the CaCO3 nanocrystals.
Therefore, bio-based CaCO3 nanocrystals were shown to stimulate osteoblast differentiation and improve the osteointegration process.
American Psychological Association (APA)
Shafiu Kamba, Abdullahi& Zakaria, Zuki Abu Bakar. 2014. Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-455182
Modern Language Association (MLA)
Shafiu Kamba, Abdullahi& Zakaria, Zuki Abu Bakar. Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-455182
American Medical Association (AMA)
Shafiu Kamba, Abdullahi& Zakaria, Zuki Abu Bakar. Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-455182
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455182